Review



ctgf standard protein detection kit  (PeproTech)


Bioz Verified Symbol PeproTech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    PeproTech ctgf standard protein detection kit
    The expression <t>of</t> <t>CNTF,</t> and <t>CTGF</t> in SCs and OECs. A, qRT-PCR analysis of purified SCs and OECs demonstrated the significant upregulation of CTGF mRNA in SCs compared with OECs. B, ELISA demonstrated greater levels of CTGF in SCM compared with OCM and ACM and heat-treated SCM (h.SCM). There was no significant difference in secreted levels of CTGF comparing ACM, OCM, and h.SCM. C, ACM contained the greatest amount of the promyelinating factor CNTF, compared with OCM or SCM. There were no significant differences in CNTF levels in SCM versus OCM. *p < 0.05. **p < 0.01. n = 3 biological replicates for qRT-PCR; n = 4 batches of CM for ELISA.
    Ctgf Standard Protein Detection Kit, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ctgf+standard+protein+detection+kit/ctgf+standard+protein+detection+kit/pmc06618810-100-29-34
    Average 90 stars, based on 1 article reviews
    ctgf standard protein detection kit - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Schwann Cells But Not Olfactory Ensheathing Cells Inhibit CNS Myelination via the Secretion of Connective Tissue Growth Factor"

    Article Title: Schwann Cells But Not Olfactory Ensheathing Cells Inhibit CNS Myelination via the Secretion of Connective Tissue Growth Factor

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.3233-13.2013

    The expression of CNTF, and CTGF in SCs and OECs. A, qRT-PCR analysis of purified SCs and OECs demonstrated the significant upregulation of CTGF mRNA in SCs compared with OECs. B, ELISA demonstrated greater levels of CTGF in SCM compared with OCM and ACM and heat-treated SCM (h.SCM). There was no significant difference in secreted levels of CTGF comparing ACM, OCM, and h.SCM. C, ACM contained the greatest amount of the promyelinating factor CNTF, compared with OCM or SCM. There were no significant differences in CNTF levels in SCM versus OCM. *p < 0.05. **p < 0.01. n = 3 biological replicates for qRT-PCR; n = 4 batches of CM for ELISA.
    Figure Legend Snippet: The expression of CNTF, and CTGF in SCs and OECs. A, qRT-PCR analysis of purified SCs and OECs demonstrated the significant upregulation of CTGF mRNA in SCs compared with OECs. B, ELISA demonstrated greater levels of CTGF in SCM compared with OCM and ACM and heat-treated SCM (h.SCM). There was no significant difference in secreted levels of CTGF comparing ACM, OCM, and h.SCM. C, ACM contained the greatest amount of the promyelinating factor CNTF, compared with OCM or SCM. There were no significant differences in CNTF levels in SCM versus OCM. *p < 0.05. **p < 0.01. n = 3 biological replicates for qRT-PCR; n = 4 batches of CM for ELISA.

    Techniques Used: Expressing, Quantitative RT-PCR, Purification, Enzyme-linked Immunosorbent Assay

    Pretreatment (PT) of astrocyte monolayers with SCM or CTGF causes translational changes in the astrocyte and a reduction in endogenous CNS myelination. Astrocytes were pretreated everyday for 4 d with SCM or CTGF before being taken directly for analysis (A, B, D–F) or being used as a monolayer to support a myelinating culture (C). Anti-GFAP suggested morphological changes in the astrocyte akin to a reactive phenotype after SC-PT (A, B), but Western analysis demonstrated no significant changes in GFAP levels (D). However, qRT-PCR studies showed that mRNA BMP-4 was significantly upregulated in astrocytes after SC or CTGF-PT, compared with controls. Conversely, IGF-2 mRNA was significantly downregulated after both types of treatment. CTGF mRNA was upregulated in astrocytes after CTGF-PT compared with both SC-PT and untreated controls (E), which was also confirmed by ELISA (F). Pretreated astrocytes not rinsed free (unwashed) of CTGF had significantly higher levels of CTGF in ACM compared with other conditions (F). The above findings correlated with a significant decrease in the level of oligodendrocyte myelination in culture after SC-PT of the astrocyte monolayer (C). Anti-GFAP labeled astrocytes, whereas DAPI labeled nuclei. Myelin was quantified using SMI-31 to label neurites and anti-PLP to label myelin sheaths and oligodendrocytes. n = 3. *p < 0.05. **p < 0.01. Scale bar, 50 μm.
    Figure Legend Snippet: Pretreatment (PT) of astrocyte monolayers with SCM or CTGF causes translational changes in the astrocyte and a reduction in endogenous CNS myelination. Astrocytes were pretreated everyday for 4 d with SCM or CTGF before being taken directly for analysis (A, B, D–F) or being used as a monolayer to support a myelinating culture (C). Anti-GFAP suggested morphological changes in the astrocyte akin to a reactive phenotype after SC-PT (A, B), but Western analysis demonstrated no significant changes in GFAP levels (D). However, qRT-PCR studies showed that mRNA BMP-4 was significantly upregulated in astrocytes after SC or CTGF-PT, compared with controls. Conversely, IGF-2 mRNA was significantly downregulated after both types of treatment. CTGF mRNA was upregulated in astrocytes after CTGF-PT compared with both SC-PT and untreated controls (E), which was also confirmed by ELISA (F). Pretreated astrocytes not rinsed free (unwashed) of CTGF had significantly higher levels of CTGF in ACM compared with other conditions (F). The above findings correlated with a significant decrease in the level of oligodendrocyte myelination in culture after SC-PT of the astrocyte monolayer (C). Anti-GFAP labeled astrocytes, whereas DAPI labeled nuclei. Myelin was quantified using SMI-31 to label neurites and anti-PLP to label myelin sheaths and oligodendrocytes. n = 3. *p < 0.05. **p < 0.01. Scale bar, 50 μm.

    Techniques Used: Western Blot, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Labeling

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Schwann Cells But Not Olfactory Ensheathing Cells Inhibit CNS Myelination via the Secretion of Connective Tissue Growth Factor
    Article Snippet: Forward and reverse primers for connective tissue growth factor (CTGF/CCN2), bone morphogenic protein 4 (BMP4) and insulin growth factor 2 (IGF2) were bought from Primer Design and validated for use in rat cultures. . ELISA. .. To assess the levels of CNTF or CTGF in CM from SCs, OECs, and astrocytes, ELISA kits were used according to the manufacturer's instructions (CNTF DuoSet ELISA Kit, RayBiotech; CTGF standard protein detection kit, PeproTech). .. The standards and samples were added to the wells in triplicate, and the plate was read using a spectrophotometer (Dynex Technologies; MRX 2.02) and Revelation software (version 4.25) to determine the optical density of each well.

    Article Title: Schwann Cells But Not Olfactory Ensheathing Cells Inhibit CNS Myelination via the Secretion of Connective Tissue Growth Factor
    Article Snippet: .. To assess the levels of CNTF or CTGF in CM from SCs, OECs, and astrocytes, ELISA kits were used according to the manufacturer’s instructions (CNTF DuoSet ELISA Kit, RayBiotech; CTGF standard protein detection kit, PeproTech). .. The standards and samples were added to the wells in triplicate, and the plate was read using a spectrophotometer (Dynex Technologies; MRX 2.02) and Revelation software (version 4.25) to determine the optical density of each well.

    other:

    Article Title: Schwann Cells But Not Olfactory Ensheathing Cells Inhibit CNS Myelination via the Secretion of Connective Tissue Growth Factor
    Article Snippet: The cells were then scraped off the coverslip, spun, and total protein concentration for each sample measured using the Nanodrop (Invitrogen).



    Similar Products

    ctgf standard protein detection kit  (PeproTech)
    90
    PeproTech ctgf standard protein detection kit
    The expression <t>of</t> <t>CNTF,</t> and <t>CTGF</t> in SCs and OECs. A, qRT-PCR analysis of purified SCs and OECs demonstrated the significant upregulation of CTGF mRNA in SCs compared with OECs. B, ELISA demonstrated greater levels of CTGF in SCM compared with OCM and ACM and heat-treated SCM (h.SCM). There was no significant difference in secreted levels of CTGF comparing ACM, OCM, and h.SCM. C, ACM contained the greatest amount of the promyelinating factor CNTF, compared with OCM or SCM. There were no significant differences in CNTF levels in SCM versus OCM. *p < 0.05. **p < 0.01. n = 3 biological replicates for qRT-PCR; n = 4 batches of CM for ELISA.
    Ctgf Standard Protein Detection Kit, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ctgf+standard+protein+detection+kit/ctgf+standard+protein+detection+kit/pmc06618810-100-29-34
    Average 90 stars, based on 1 article reviews
    ctgf standard protein detection kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    The expression of CNTF, and CTGF in SCs and OECs. A, qRT-PCR analysis of purified SCs and OECs demonstrated the significant upregulation of CTGF mRNA in SCs compared with OECs. B, ELISA demonstrated greater levels of CTGF in SCM compared with OCM and ACM and heat-treated SCM (h.SCM). There was no significant difference in secreted levels of CTGF comparing ACM, OCM, and h.SCM. C, ACM contained the greatest amount of the promyelinating factor CNTF, compared with OCM or SCM. There were no significant differences in CNTF levels in SCM versus OCM. *p < 0.05. **p < 0.01. n = 3 biological replicates for qRT-PCR; n = 4 batches of CM for ELISA.

    Journal: The Journal of Neuroscience

    Article Title: Schwann Cells But Not Olfactory Ensheathing Cells Inhibit CNS Myelination via the Secretion of Connective Tissue Growth Factor

    doi: 10.1523/JNEUROSCI.3233-13.2013

    Figure Lengend Snippet: The expression of CNTF, and CTGF in SCs and OECs. A, qRT-PCR analysis of purified SCs and OECs demonstrated the significant upregulation of CTGF mRNA in SCs compared with OECs. B, ELISA demonstrated greater levels of CTGF in SCM compared with OCM and ACM and heat-treated SCM (h.SCM). There was no significant difference in secreted levels of CTGF comparing ACM, OCM, and h.SCM. C, ACM contained the greatest amount of the promyelinating factor CNTF, compared with OCM or SCM. There were no significant differences in CNTF levels in SCM versus OCM. *p < 0.05. **p < 0.01. n = 3 biological replicates for qRT-PCR; n = 4 batches of CM for ELISA.

    Article Snippet: To assess the levels of CNTF or CTGF in CM from SCs, OECs, and astrocytes, ELISA kits were used according to the manufacturer's instructions (CNTF DuoSet ELISA Kit, RayBiotech; CTGF standard protein detection kit, PeproTech).

    Techniques: Expressing, Quantitative RT-PCR, Purification, Enzyme-linked Immunosorbent Assay

    Pretreatment (PT) of astrocyte monolayers with SCM or CTGF causes translational changes in the astrocyte and a reduction in endogenous CNS myelination. Astrocytes were pretreated everyday for 4 d with SCM or CTGF before being taken directly for analysis (A, B, D–F) or being used as a monolayer to support a myelinating culture (C). Anti-GFAP suggested morphological changes in the astrocyte akin to a reactive phenotype after SC-PT (A, B), but Western analysis demonstrated no significant changes in GFAP levels (D). However, qRT-PCR studies showed that mRNA BMP-4 was significantly upregulated in astrocytes after SC or CTGF-PT, compared with controls. Conversely, IGF-2 mRNA was significantly downregulated after both types of treatment. CTGF mRNA was upregulated in astrocytes after CTGF-PT compared with both SC-PT and untreated controls (E), which was also confirmed by ELISA (F). Pretreated astrocytes not rinsed free (unwashed) of CTGF had significantly higher levels of CTGF in ACM compared with other conditions (F). The above findings correlated with a significant decrease in the level of oligodendrocyte myelination in culture after SC-PT of the astrocyte monolayer (C). Anti-GFAP labeled astrocytes, whereas DAPI labeled nuclei. Myelin was quantified using SMI-31 to label neurites and anti-PLP to label myelin sheaths and oligodendrocytes. n = 3. *p < 0.05. **p < 0.01. Scale bar, 50 μm.

    Journal: The Journal of Neuroscience

    Article Title: Schwann Cells But Not Olfactory Ensheathing Cells Inhibit CNS Myelination via the Secretion of Connective Tissue Growth Factor

    doi: 10.1523/JNEUROSCI.3233-13.2013

    Figure Lengend Snippet: Pretreatment (PT) of astrocyte monolayers with SCM or CTGF causes translational changes in the astrocyte and a reduction in endogenous CNS myelination. Astrocytes were pretreated everyday for 4 d with SCM or CTGF before being taken directly for analysis (A, B, D–F) or being used as a monolayer to support a myelinating culture (C). Anti-GFAP suggested morphological changes in the astrocyte akin to a reactive phenotype after SC-PT (A, B), but Western analysis demonstrated no significant changes in GFAP levels (D). However, qRT-PCR studies showed that mRNA BMP-4 was significantly upregulated in astrocytes after SC or CTGF-PT, compared with controls. Conversely, IGF-2 mRNA was significantly downregulated after both types of treatment. CTGF mRNA was upregulated in astrocytes after CTGF-PT compared with both SC-PT and untreated controls (E), which was also confirmed by ELISA (F). Pretreated astrocytes not rinsed free (unwashed) of CTGF had significantly higher levels of CTGF in ACM compared with other conditions (F). The above findings correlated with a significant decrease in the level of oligodendrocyte myelination in culture after SC-PT of the astrocyte monolayer (C). Anti-GFAP labeled astrocytes, whereas DAPI labeled nuclei. Myelin was quantified using SMI-31 to label neurites and anti-PLP to label myelin sheaths and oligodendrocytes. n = 3. *p < 0.05. **p < 0.01. Scale bar, 50 μm.

    Article Snippet: To assess the levels of CNTF or CTGF in CM from SCs, OECs, and astrocytes, ELISA kits were used according to the manufacturer's instructions (CNTF DuoSet ELISA Kit, RayBiotech; CTGF standard protein detection kit, PeproTech).

    Techniques: Western Blot, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Labeling